ets 1 Search Results


anti ets1  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology anti ets1
Anti Ets1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ets+1/10__1158_slash_1541___7786__mcr___08___0229-238-15-16?v=Santa+Cruz+Biotechnology
Average 95 stars, based on 1 article reviews
anti ets1 - by Bioz Stars, 2026-07
95/100 stars
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exogenous ets1 complementary dna  (OriGene)
90
OriGene exogenous ets1 complementary dna
Exogenous Ets1 Complementary Dna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ets+1/pm26455323-162-11-15?v=OriGene
Average 90 stars, based on 1 article reviews
exogenous ets1 complementary dna - by Bioz Stars, 2026-07
90/100 stars
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mouse ets1 sirna  (OriGene)
90
OriGene mouse ets1 sirna
Mouse Ets1 Sirna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ets+1/10__1161_slash_atvbaha__116__308794-289-10-13?v=OriGene
Average 90 stars, based on 1 article reviews
mouse ets1 sirna - by Bioz Stars, 2026-07
90/100 stars
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human ets 1 cdna  (OriGene)
90
OriGene human ets 1 cdna
Human Ets 1 Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ets+1/pmc08168518-33-0-6?v=OriGene
Average 90 stars, based on 1 article reviews
human ets 1 cdna - by Bioz Stars, 2026-07
90/100 stars
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ets1 sirnas  (OriGene)
90
OriGene ets1 sirnas
Figure 3 | ANGPTL2 enhances CXCR4 and <t>ETS1</t> expression in MDA-MB231 cells. (A) Representative image showing immunoblot of ETS1 and HSC70 in MB231/miANGPTL2 and MB231/miLacZ cells. Full-length blots are presented in Supplementary Fig. S7A. (B) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/miLacZ was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t- test). (C) Representative immunoblot of ETS1 and HSC70 in MB231/Control and MB231/ANGPTL2 cells. Full-length blots are shown in Supplementary Fig. S7D. (D) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t-test). (E) Relative ANGPTL2 and CXCR4 expression in MB231/ANGPTL2 cells. Data are means 6 SEM from three experiments; wwP , 0.01(unpaired two-tailed Student’s t-test). (F) CXCR4 cell surface expression in MB231/ANGPTL2 and MB231/Control cells based on flow cytometry. Gray shaded area represents isotype control antibody group. MB231/ANGPTL2 cells, dotted-line; MB231/Control cells, solid line. (G) Representative image and analysis of immunoblotting for ETS1 relative to HSC70 in MB231/ANGPTL2 cells transduced with ETS1 siRNA. Full- length blots are presented in Supplementary Fig. S8. (H) Quantitative ETS1 protein levels relative to HSC70. Control siRNA cells, black bar; ETS1 siRNA- 1 cells, blue; and ETS1 siRNA-2 cells, red. Data from Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05, wwP , 0.01 (unpaired two-tailed Student’s t-test) (I) CXCR4 cell surface expression in Control siRNA, ETS1 siRNA-1, and ETS1 siRNA-2 cells based on flow cytometry. Gray shaded area represents isotype control antibody group. Control siRNA cells, black line; ETS1 siRNA-1, blue; and ETS1 siRNA-2, red.
Ets1 Sirnas, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ets+1/pm25773070-257-10-15?v=OriGene
Average 90 stars, based on 1 article reviews
ets1 sirnas - by Bioz Stars, 2026-07
90/100 stars
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rabbit anti phospho ets 1 thr38 antibody  (Novus Biologicals)
90
Novus Biologicals rabbit anti phospho ets 1 thr38 antibody
Figure 3 | ANGPTL2 enhances CXCR4 and <t>ETS1</t> expression in MDA-MB231 cells. (A) Representative image showing immunoblot of ETS1 and HSC70 in MB231/miANGPTL2 and MB231/miLacZ cells. Full-length blots are presented in Supplementary Fig. S7A. (B) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/miLacZ was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t- test). (C) Representative immunoblot of ETS1 and HSC70 in MB231/Control and MB231/ANGPTL2 cells. Full-length blots are shown in Supplementary Fig. S7D. (D) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t-test). (E) Relative ANGPTL2 and CXCR4 expression in MB231/ANGPTL2 cells. Data are means 6 SEM from three experiments; wwP , 0.01(unpaired two-tailed Student’s t-test). (F) CXCR4 cell surface expression in MB231/ANGPTL2 and MB231/Control cells based on flow cytometry. Gray shaded area represents isotype control antibody group. MB231/ANGPTL2 cells, dotted-line; MB231/Control cells, solid line. (G) Representative image and analysis of immunoblotting for ETS1 relative to HSC70 in MB231/ANGPTL2 cells transduced with ETS1 siRNA. Full- length blots are presented in Supplementary Fig. S8. (H) Quantitative ETS1 protein levels relative to HSC70. Control siRNA cells, black bar; ETS1 siRNA- 1 cells, blue; and ETS1 siRNA-2 cells, red. Data from Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05, wwP , 0.01 (unpaired two-tailed Student’s t-test) (I) CXCR4 cell surface expression in Control siRNA, ETS1 siRNA-1, and ETS1 siRNA-2 cells based on flow cytometry. Gray shaded area represents isotype control antibody group. Control siRNA cells, black line; ETS1 siRNA-1, blue; and ETS1 siRNA-2, red.
Rabbit Anti Phospho Ets 1 Thr38 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ets+1/pm27808345-117-37-41?v=Novus+Biologicals
Average 90 stars, based on 1 article reviews
rabbit anti phospho ets 1 thr38 antibody - by Bioz Stars, 2026-07
90/100 stars
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anti runt related transcription factor runx 2  (Boster Bio)
92
Boster Bio anti runt related transcription factor runx 2
Figure 3 | ANGPTL2 enhances CXCR4 and <t>ETS1</t> expression in MDA-MB231 cells. (A) Representative image showing immunoblot of ETS1 and HSC70 in MB231/miANGPTL2 and MB231/miLacZ cells. Full-length blots are presented in Supplementary Fig. S7A. (B) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/miLacZ was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t- test). (C) Representative immunoblot of ETS1 and HSC70 in MB231/Control and MB231/ANGPTL2 cells. Full-length blots are shown in Supplementary Fig. S7D. (D) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t-test). (E) Relative ANGPTL2 and CXCR4 expression in MB231/ANGPTL2 cells. Data are means 6 SEM from three experiments; wwP , 0.01(unpaired two-tailed Student’s t-test). (F) CXCR4 cell surface expression in MB231/ANGPTL2 and MB231/Control cells based on flow cytometry. Gray shaded area represents isotype control antibody group. MB231/ANGPTL2 cells, dotted-line; MB231/Control cells, solid line. (G) Representative image and analysis of immunoblotting for ETS1 relative to HSC70 in MB231/ANGPTL2 cells transduced with ETS1 siRNA. Full- length blots are presented in Supplementary Fig. S8. (H) Quantitative ETS1 protein levels relative to HSC70. Control siRNA cells, black bar; ETS1 siRNA- 1 cells, blue; and ETS1 siRNA-2 cells, red. Data from Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05, wwP , 0.01 (unpaired two-tailed Student’s t-test) (I) CXCR4 cell surface expression in Control siRNA, ETS1 siRNA-1, and ETS1 siRNA-2 cells based on flow cytometry. Gray shaded area represents isotype control antibody group. Control siRNA cells, black line; ETS1 siRNA-1, blue; and ETS1 siRNA-2, red.
Anti Runt Related Transcription Factor Runx 2, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ets+1/pmc12835961-64-29-37?v=Boster+Bio
Average 92 stars, based on 1 article reviews
anti runt related transcription factor runx 2 - by Bioz Stars, 2026-07
92/100 stars
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ets1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology ets1
Figure 3 | ANGPTL2 enhances CXCR4 and <t>ETS1</t> expression in MDA-MB231 cells. (A) Representative image showing immunoblot of ETS1 and HSC70 in MB231/miANGPTL2 and MB231/miLacZ cells. Full-length blots are presented in Supplementary Fig. S7A. (B) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/miLacZ was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t- test). (C) Representative immunoblot of ETS1 and HSC70 in MB231/Control and MB231/ANGPTL2 cells. Full-length blots are shown in Supplementary Fig. S7D. (D) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t-test). (E) Relative ANGPTL2 and CXCR4 expression in MB231/ANGPTL2 cells. Data are means 6 SEM from three experiments; wwP , 0.01(unpaired two-tailed Student’s t-test). (F) CXCR4 cell surface expression in MB231/ANGPTL2 and MB231/Control cells based on flow cytometry. Gray shaded area represents isotype control antibody group. MB231/ANGPTL2 cells, dotted-line; MB231/Control cells, solid line. (G) Representative image and analysis of immunoblotting for ETS1 relative to HSC70 in MB231/ANGPTL2 cells transduced with ETS1 siRNA. Full- length blots are presented in Supplementary Fig. S8. (H) Quantitative ETS1 protein levels relative to HSC70. Control siRNA cells, black bar; ETS1 siRNA- 1 cells, blue; and ETS1 siRNA-2 cells, red. Data from Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05, wwP , 0.01 (unpaired two-tailed Student’s t-test) (I) CXCR4 cell surface expression in Control siRNA, ETS1 siRNA-1, and ETS1 siRNA-2 cells based on flow cytometry. Gray shaded area represents isotype control antibody group. Control siRNA cells, black line; ETS1 siRNA-1, blue; and ETS1 siRNA-2, red.
Ets1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ets+1/10__1158_slash_1541___7786__mcr___17___0433-108-10-20?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
ets1 - by Bioz Stars, 2026-07
93/100 stars
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ets1 conditional knock  (Cyagen Biosciences)
92
Cyagen Biosciences ets1 conditional knock
Figure 3 | ANGPTL2 enhances CXCR4 and <t>ETS1</t> expression in MDA-MB231 cells. (A) Representative image showing immunoblot of ETS1 and HSC70 in MB231/miANGPTL2 and MB231/miLacZ cells. Full-length blots are presented in Supplementary Fig. S7A. (B) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/miLacZ was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t- test). (C) Representative immunoblot of ETS1 and HSC70 in MB231/Control and MB231/ANGPTL2 cells. Full-length blots are shown in Supplementary Fig. S7D. (D) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t-test). (E) Relative ANGPTL2 and CXCR4 expression in MB231/ANGPTL2 cells. Data are means 6 SEM from three experiments; wwP , 0.01(unpaired two-tailed Student’s t-test). (F) CXCR4 cell surface expression in MB231/ANGPTL2 and MB231/Control cells based on flow cytometry. Gray shaded area represents isotype control antibody group. MB231/ANGPTL2 cells, dotted-line; MB231/Control cells, solid line. (G) Representative image and analysis of immunoblotting for ETS1 relative to HSC70 in MB231/ANGPTL2 cells transduced with ETS1 siRNA. Full- length blots are presented in Supplementary Fig. S8. (H) Quantitative ETS1 protein levels relative to HSC70. Control siRNA cells, black bar; ETS1 siRNA- 1 cells, blue; and ETS1 siRNA-2 cells, red. Data from Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05, wwP , 0.01 (unpaired two-tailed Student’s t-test) (I) CXCR4 cell surface expression in Control siRNA, ETS1 siRNA-1, and ETS1 siRNA-2 cells based on flow cytometry. Gray shaded area represents isotype control antibody group. Control siRNA cells, black line; ETS1 siRNA-1, blue; and ETS1 siRNA-2, red.
Ets1 Conditional Knock, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ets+1/pmc10883921__41467_2024_45899_MOESM2_ESM-42-15-22?v=Cyagen+Biosciences
Average 92 stars, based on 1 article reviews
ets1 conditional knock - by Bioz Stars, 2026-07
92/100 stars
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human cdna  (OriGene)
90
OriGene human cdna
Figure 3 | ANGPTL2 enhances CXCR4 and <t>ETS1</t> expression in MDA-MB231 cells. (A) Representative image showing immunoblot of ETS1 and HSC70 in MB231/miANGPTL2 and MB231/miLacZ cells. Full-length blots are presented in Supplementary Fig. S7A. (B) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/miLacZ was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t- test). (C) Representative immunoblot of ETS1 and HSC70 in MB231/Control and MB231/ANGPTL2 cells. Full-length blots are shown in Supplementary Fig. S7D. (D) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t-test). (E) Relative ANGPTL2 and CXCR4 expression in MB231/ANGPTL2 cells. Data are means 6 SEM from three experiments; wwP , 0.01(unpaired two-tailed Student’s t-test). (F) CXCR4 cell surface expression in MB231/ANGPTL2 and MB231/Control cells based on flow cytometry. Gray shaded area represents isotype control antibody group. MB231/ANGPTL2 cells, dotted-line; MB231/Control cells, solid line. (G) Representative image and analysis of immunoblotting for ETS1 relative to HSC70 in MB231/ANGPTL2 cells transduced with ETS1 siRNA. Full- length blots are presented in Supplementary Fig. S8. (H) Quantitative ETS1 protein levels relative to HSC70. Control siRNA cells, black bar; ETS1 siRNA- 1 cells, blue; and ETS1 siRNA-2 cells, red. Data from Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05, wwP , 0.01 (unpaired two-tailed Student’s t-test) (I) CXCR4 cell surface expression in Control siRNA, ETS1 siRNA-1, and ETS1 siRNA-2 cells based on flow cytometry. Gray shaded area represents isotype control antibody group. Control siRNA cells, black line; ETS1 siRNA-1, blue; and ETS1 siRNA-2, red.
Human Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ets+1/pmc07239993-27-5-10?v=OriGene
Average 90 stars, based on 1 article reviews
human cdna - by Bioz Stars, 2026-07
90/100 stars
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anti c ets 1 2  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology anti c ets 1 2
Figure 3 | ANGPTL2 enhances CXCR4 and <t>ETS1</t> expression in MDA-MB231 cells. (A) Representative image showing immunoblot of ETS1 and HSC70 in MB231/miANGPTL2 and MB231/miLacZ cells. Full-length blots are presented in Supplementary Fig. S7A. (B) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/miLacZ was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t- test). (C) Representative immunoblot of ETS1 and HSC70 in MB231/Control and MB231/ANGPTL2 cells. Full-length blots are shown in Supplementary Fig. S7D. (D) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t-test). (E) Relative ANGPTL2 and CXCR4 expression in MB231/ANGPTL2 cells. Data are means 6 SEM from three experiments; wwP , 0.01(unpaired two-tailed Student’s t-test). (F) CXCR4 cell surface expression in MB231/ANGPTL2 and MB231/Control cells based on flow cytometry. Gray shaded area represents isotype control antibody group. MB231/ANGPTL2 cells, dotted-line; MB231/Control cells, solid line. (G) Representative image and analysis of immunoblotting for ETS1 relative to HSC70 in MB231/ANGPTL2 cells transduced with ETS1 siRNA. Full- length blots are presented in Supplementary Fig. S8. (H) Quantitative ETS1 protein levels relative to HSC70. Control siRNA cells, black bar; ETS1 siRNA- 1 cells, blue; and ETS1 siRNA-2 cells, red. Data from Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05, wwP , 0.01 (unpaired two-tailed Student’s t-test) (I) CXCR4 cell surface expression in Control siRNA, ETS1 siRNA-1, and ETS1 siRNA-2 cells based on flow cytometry. Gray shaded area represents isotype control antibody group. Control siRNA cells, black line; ETS1 siRNA-1, blue; and ETS1 siRNA-2, red.
Anti C Ets 1 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ets+1/pmc02626199-362-6-16?v=Santa+Cruz+Biotechnology
Average 92 stars, based on 1 article reviews
anti c ets 1 2 - by Bioz Stars, 2026-07
92/100 stars
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cmv promoter  (OriGene)
93
OriGene cmv promoter
Figure 3 | ANGPTL2 enhances CXCR4 and <t>ETS1</t> expression in MDA-MB231 cells. (A) Representative image showing immunoblot of ETS1 and HSC70 in MB231/miANGPTL2 and MB231/miLacZ cells. Full-length blots are presented in Supplementary Fig. S7A. (B) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/miLacZ was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t- test). (C) Representative immunoblot of ETS1 and HSC70 in MB231/Control and MB231/ANGPTL2 cells. Full-length blots are shown in Supplementary Fig. S7D. (D) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t-test). (E) Relative ANGPTL2 and CXCR4 expression in MB231/ANGPTL2 cells. Data are means 6 SEM from three experiments; wwP , 0.01(unpaired two-tailed Student’s t-test). (F) CXCR4 cell surface expression in MB231/ANGPTL2 and MB231/Control cells based on flow cytometry. Gray shaded area represents isotype control antibody group. MB231/ANGPTL2 cells, dotted-line; MB231/Control cells, solid line. (G) Representative image and analysis of immunoblotting for ETS1 relative to HSC70 in MB231/ANGPTL2 cells transduced with ETS1 siRNA. Full- length blots are presented in Supplementary Fig. S8. (H) Quantitative ETS1 protein levels relative to HSC70. Control siRNA cells, black bar; ETS1 siRNA- 1 cells, blue; and ETS1 siRNA-2 cells, red. Data from Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05, wwP , 0.01 (unpaired two-tailed Student’s t-test) (I) CXCR4 cell surface expression in Control siRNA, ETS1 siRNA-1, and ETS1 siRNA-2 cells based on flow cytometry. Gray shaded area represents isotype control antibody group. Control siRNA cells, black line; ETS1 siRNA-1, blue; and ETS1 siRNA-2, red.
Cmv Promoter, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ets+1/us12460225-244-12-17?v=OriGene
Average 93 stars, based on 1 article reviews
cmv promoter - by Bioz Stars, 2026-07
93/100 stars
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Image Search Results


Figure 3 | ANGPTL2 enhances CXCR4 and ETS1 expression in MDA-MB231 cells. (A) Representative image showing immunoblot of ETS1 and HSC70 in MB231/miANGPTL2 and MB231/miLacZ cells. Full-length blots are presented in Supplementary Fig. S7A. (B) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/miLacZ was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t- test). (C) Representative immunoblot of ETS1 and HSC70 in MB231/Control and MB231/ANGPTL2 cells. Full-length blots are shown in Supplementary Fig. S7D. (D) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t-test). (E) Relative ANGPTL2 and CXCR4 expression in MB231/ANGPTL2 cells. Data are means 6 SEM from three experiments; wwP , 0.01(unpaired two-tailed Student’s t-test). (F) CXCR4 cell surface expression in MB231/ANGPTL2 and MB231/Control cells based on flow cytometry. Gray shaded area represents isotype control antibody group. MB231/ANGPTL2 cells, dotted-line; MB231/Control cells, solid line. (G) Representative image and analysis of immunoblotting for ETS1 relative to HSC70 in MB231/ANGPTL2 cells transduced with ETS1 siRNA. Full- length blots are presented in Supplementary Fig. S8. (H) Quantitative ETS1 protein levels relative to HSC70. Control siRNA cells, black bar; ETS1 siRNA- 1 cells, blue; and ETS1 siRNA-2 cells, red. Data from Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05, wwP , 0.01 (unpaired two-tailed Student’s t-test) (I) CXCR4 cell surface expression in Control siRNA, ETS1 siRNA-1, and ETS1 siRNA-2 cells based on flow cytometry. Gray shaded area represents isotype control antibody group. Control siRNA cells, black line; ETS1 siRNA-1, blue; and ETS1 siRNA-2, red.

Journal: Scientific reports

Article Title: ANGPTL2 increases bone metastasis of breast cancer cells through enhancing CXCR4 signaling.

doi: 10.1038/srep09170

Figure Lengend Snippet: Figure 3 | ANGPTL2 enhances CXCR4 and ETS1 expression in MDA-MB231 cells. (A) Representative image showing immunoblot of ETS1 and HSC70 in MB231/miANGPTL2 and MB231/miLacZ cells. Full-length blots are presented in Supplementary Fig. S7A. (B) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/miLacZ was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t- test). (C) Representative immunoblot of ETS1 and HSC70 in MB231/Control and MB231/ANGPTL2 cells. Full-length blots are shown in Supplementary Fig. S7D. (D) Quantitative ETS1 protein levels relative to HSC70. Data from MB231/Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05 (unpaired two-tailed Student’s t-test). (E) Relative ANGPTL2 and CXCR4 expression in MB231/ANGPTL2 cells. Data are means 6 SEM from three experiments; wwP , 0.01(unpaired two-tailed Student’s t-test). (F) CXCR4 cell surface expression in MB231/ANGPTL2 and MB231/Control cells based on flow cytometry. Gray shaded area represents isotype control antibody group. MB231/ANGPTL2 cells, dotted-line; MB231/Control cells, solid line. (G) Representative image and analysis of immunoblotting for ETS1 relative to HSC70 in MB231/ANGPTL2 cells transduced with ETS1 siRNA. Full- length blots are presented in Supplementary Fig. S8. (H) Quantitative ETS1 protein levels relative to HSC70. Control siRNA cells, black bar; ETS1 siRNA- 1 cells, blue; and ETS1 siRNA-2 cells, red. Data from Control was set at 1. Data are means 6 SEM from three experiments; wP , 0.05, wwP , 0.01 (unpaired two-tailed Student’s t-test) (I) CXCR4 cell surface expression in Control siRNA, ETS1 siRNA-1, and ETS1 siRNA-2 cells based on flow cytometry. Gray shaded area represents isotype control antibody group. Control siRNA cells, black line; ETS1 siRNA-1, blue; and ETS1 siRNA-2, red.

Article Snippet: MB231/ANGPTL2 cells were reseeded in 6-well plates with two specific ETS1 siRNAs (SR301470A and SR301470B; ORIGENE, ETS1 (Locus ID 2113) Trilencer-27 Human siRNA) or control Trilencer-27 Universal Scrambled Negative Control (ORIGENE, Rockville, MD, USA).

Techniques: Expressing, Western Blot, Two Tailed Test, Control, Flow Cytometry, Transduction

Figure 6 | Mechanistic model of crosstalk between ANGPTL2 and CXCL12-activated CXCR4 signaling in breast cancer metastasis to bone. (A) Tumor cell-derived ANGPTL2 increases CXCR4 expression in an autocrine manner, potentially through ETS1 up-regulation, resulting in increased metastasis of tumor cells to bone, a region of high expression of the CXCR4 ligand CXCL12. (B) In bone, CXCL12-activated CXCR4 signaling increases MMP-13 expression and accelerates osteolysis and bone engraftment.

Journal: Scientific reports

Article Title: ANGPTL2 increases bone metastasis of breast cancer cells through enhancing CXCR4 signaling.

doi: 10.1038/srep09170

Figure Lengend Snippet: Figure 6 | Mechanistic model of crosstalk between ANGPTL2 and CXCL12-activated CXCR4 signaling in breast cancer metastasis to bone. (A) Tumor cell-derived ANGPTL2 increases CXCR4 expression in an autocrine manner, potentially through ETS1 up-regulation, resulting in increased metastasis of tumor cells to bone, a region of high expression of the CXCR4 ligand CXCL12. (B) In bone, CXCL12-activated CXCR4 signaling increases MMP-13 expression and accelerates osteolysis and bone engraftment.

Article Snippet: MB231/ANGPTL2 cells were reseeded in 6-well plates with two specific ETS1 siRNAs (SR301470A and SR301470B; ORIGENE, ETS1 (Locus ID 2113) Trilencer-27 Human siRNA) or control Trilencer-27 Universal Scrambled Negative Control (ORIGENE, Rockville, MD, USA).

Techniques: Derivative Assay, Expressing